How to use this site
Human Chromosome 21 gene expression atlas web site collects data derived from
a systematic study on chromosome 21 genes. This study identifies the tissues
where each chromosome 21 gene is turned on during development and in the adult.
It is based on the expression analysis of all identifiable murine orthologues
of human chromosome 21 genes by RNA in situ hybridization on
whole-mounts (E9.5, E10.5 embryos) and tissue sections (E14.5 embryos), and by
RT-PCR on adult tissue.
the Scientific Data section, you can access:
Summary table containing all the expression data (see below);
- Examples of gene expression in specific tissues, with images of expression of some genes in
organs affected in Down Syndrome patients, i.e., Pancreas, Gut,
Limb, Heart, Thymus, Cerebellum;
and Methods with a description of the techniques used for these
table shows all the identified genes on chromosome 21 ordered by their position
on human chromosome 21. The third column lists the Human gene accession number
and the fourth column the accession number of the murine ortholog gene.
Finally, the last column on the right summarizes the Status in the mouse. By
clicking on Status in mouse a description of the color code will be
found. To access the data for each gene you need to click on the gene name.
This will take you to a gene card.
Gene characteristics button will link you to a public database
(LocusLink or Genecard) collecting all the available information on this
button Probe will open a table with 25 genes among which the gene
of interest is listed. The table reports the information on the probe used
for the expression studies.
- The RT-PCR button
opens a table of 25 genes among which the gene of interest is listed. This
table reports the primers used for RT-PCR studies and the results obtained
using RNA from adult murine tissues such as: brain, heart, kidney, thymus, liver, stomach,
muscle, lung, testis, ovary, skin, eyes. RT-PCR data obtained from
embryonic RNA (E8.5, E9.5, E12.5, E19.5) is also shown. + was used to
report expression in that tissue, no detectable expression is blank.
- By clicking on E9.5 or the small image
you can open the image of an E9.5 embryo hybridized with the probe for the
gene of interest. Similarly, by clicking on E10.5 or E14.5 you
can access to bigger images of the embryos.
- Using the WholeMountTable button you will open a
table with 25 genes among which the
gene of interest is listed. This table summarizes the data obtained from
whole mount in situ hybridization experiments using + when the
gene was detected in a tissue and ++ for strong signal. The tissues
listed in this table are: brain, spinal chord
(SC), eyes, otocyst, dorsal root ganglia (DRG), branchial arches, heart,
gut, kidney, limbs, skin.
- Using the E14.5MountTable button you will open a
table with 5 genes among which the
gene of interest is listed. This table summarizes the data obtained from in
situ hybridization experiments on E14.5 embryonic sections. The code
used is: (U) ubiquitous (R) regional (i)
isolated +++ strong / ++ moderate / + weak signal. The tissues
listed in this table are: forebrain, midbrain, hindbrain,
cerebellum, spinal cord, cranial ganglia, dorsal
root ganglia, parasympathetic ganglia, eye,
epithelium, cochlea, skeleton
(cartilage), lung, atrium, ventricle,
arterial system, gut,
bladder, kidney, testis, ovary, salivary, spleen, pancreas, adrenal, thyroid, thymus, liver, skin, whisker
follicles, muscle, mesenchyme, tongue, limbs,